Work Schedule
Standard (Mon-Fri)

Environmental Conditions
Able to lift 40 lbs. without assistance, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.), Will work with hazardous/toxic materials

Job Description

Thermo Fisher Scientific Inc. is seeking a highly motivated individual to join the Protein and Viral Vector Purification department as a Scientist II. This is a unique opportunity to be involved with groundbreaking research projects with the potential to make a significant impact in the scientific community. As part of a collaborative group, you will have the chance to work in a premier facility and collaborate with a diverse group of individuals who consistently demonstrate strong work behaviors.

• Conduct downstream activities including depth filtration, tangential flow filtration, and column-based purification of antibodies, non-antibody recombinant proteins, and viral vectors (primarily AAV and LV).
• Develop methods to enhance the purification of proteins and viral vectors.
• Perform analytical characterization of proteins and viral vectors, including concentration/titer, purity, aggregation, fragmentation, glycan profiles, charge variants, and biological activity.
• Troubleshoot instrumentation hardware and software related issues for FPLC and HPLCs (e.g., AKTA Pure FPLC, AKTA Avant FPLC, and Vanquish UHPLC).
• Design and conduct experiments, accurately analyze data, and interpret results.
• Collaborate with cross-functional teams to ensure the successful implementation of research projects.
• Share research findings with colleagues within the organization and present at scientific conferences.

• Strong background in protein purification/characterization with a Bachelor's degree and 4+ years of relevant academic or industry experience, a Master's degree plus 0-3+ years of experience.

• Extensive knowledge and experience in protein purification by column chromatography (affinity, IEX, NiNTA, reverse-phase, etc.).
• Proficiency in protein purification and analytical characterization using standard laboratory techniques, including AKTA-FPLC, SDS-PAGE, Western Blot, SEC-HPLC, IEX-HPLC, RP-HPLC, HIC, HILIC, affinity chromatography, ELISA, ligand-binding assays, and functional cell-based assays.
• Viral vector purification and characterization experience is a plus.
• Knowledge of Mass Spectrometry-based proteomics is a plus.
• Ability to efficiently implement technology transfer, particularly across sites.
• Ability to think creatively and apply existing analytical technologies to new applications.
• Familiarity with hardware and software for FPLC and HPLC instruments (e.g., AKTA Pure FPLC, AKTA Avant FPLC, and Vanquish UHPLC).
• Track record of completing tasks within established timelines.
• Excellent time management and multitasking capabilities.
• Proficiency in handling multiple projects and prioritizing tasks efficiently.
• Excellent verbal and written communication skills with the ability to present scientific results in a clear, concise, and accurate manner to both technical and non-technical audiences.
• Ability to generate new insights, achieve results, and address problems.