Johnson and Johnson is currently seeking an Senior Scientist, Molecular Biology located in Spring House, PA.
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The in Vivo Antibody Discovery group within Biologics Discovery is responsible for lead antibody generation from immunized animals with state-of-the-art technologies including single B cell sorting, hybridoma, display and microfluidics. In this key role, the Senior Scientist will utilize different methods including NGS to recover antibody sequences from selected hybridoma cells or single B cells. In-depth knowledge and extensive experience in molecular cloning of BCR using various approaches including RT-PCR, Topo cloning, in-fusion cloning, multiplex NGS, 10X genomics are required. A strong understanding of B-cell recovery methods such as Hybridoma technology and primary B-cell cloning will also be important. This is a critical function in a multifaceted Biologics Discovery organization committed to bringing the next generation of biotherapeutic molecules to patients. We are looking for a motivated candidate with scientific and technical expertise who can collaborate across discovery areas to bring value to our portfolio of innovative drug concepts. Proficiency to work within a highly coordinated, fast-paced environment will be a significant attribute. If this sounds like you, come join our team!
Primary responsibilities:
• Lead antibody sequence recovery from selected hits of diverse sources, including hybridoma, single B cells, immune repertoires, and in vitro display platforms.
• Execute and optimize molecular biology workflows, including Next-Generation Sequencing (NGS) and conventional Sanger sequencing, to enable efficient and accurate antibody discovery.
• Perform molecular cloning, plasmid design, and contribute to technology development within the molecular biology group to enhance discovery capabilities.
• Develop, troubleshoot, and streamline NGS workflows, identifying opportunities for process improvements to enhance sequencing accuracy and efficiency.
• Manage laboratory operations by maintaining equipment, ensuring sufficient inventory of supplies, and overseeing the routine maintenance of NGS instruments and related laboratory equipment.
• Conduct comprehensive data analysis, interpret sequencing results, and generate detailed reports. Bioinformatics experience is a plus.
• Present research findings in internal meetings, clearly communicating scientific results and providing insights into data interpretation.
• Collaborate with multidisciplinary teams across functional areas to advance therapeutic programs, contributing scientific expertise to multiple projects in a dynamic and fast-paced environment.
• Document work accurately and promptly using Electronic Lab Notebook systems (e.g., Signal) and generate protocols and SOPs.
• Work closely with stakeholders and project managers to address operational needs and ensure seamless workflow execution.
• Demonstrate scientific curiosity and problem-solving skills, contributing to innovation and continuous improvement in molecular biology and sequencing workflows.
Qualifications
Education:
• Bachelor's or Master's degree with 3-6 years of relevant experience is required
• PhD in Molecular Biology, Biomedical Engineering, Biochemistry, Bioinformatics, or a related field with 1-3 years of relevant experience in an academic or industry setting is strongly preferred
Required:
• Proficiency in molecular biology techniques, including PCR, molecular cloning, and plasmid design.
• Hands-on experience with DNA sequencing techniques (NGS, 10x Genomics, Sanger) and sequence analysis software (e.g., Geneious).
• Strong analytical and organizational skills with the ability to prioritize and manage multiple projects concurrently.
Preferred:
• Experience with applications of bioinformatics tools for sequence alignment, annotation, and variant analysis in the context of antibody discovery.
• Proven experience in analyzing and interpreting NGS data specifically related to antibody discovery including sequence diversity assessment.
• Familiarity with techniques for antibody repertoire analysis and single cell analysis.
• Knowledge of data management and visualization tools for large datasets, and knowledge with statistical analysis of NGS results.
Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.