Do you want to work with a company that tackles fascinating problems and find solutions to complex challenges? Seqirus has an exciting opportunity to join our expanding team in Holly Springs, NC. With an advanced manufacturing facility and expertise in influenza science we are one of the world leaders in the production of the influenza vaccine. We operate as one integrated global company, drawing together expert staff from different countries to collaborate. Together, we're working on the front line to protect communities from seasonal influenza and global pandemic threats. When you join Seqirus, you join a team dedicated to making a difference in people's lives. It's a feeling of possibility, creativity, and purpose that inspires us every day.
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You will be reporting to the Scientist II. You will work onsite at our CSL site in Waltham, MA location. Our benefits include an Onsite gym, cafeteria, barista coffee bar, free onsite parking and bike storage, access to Medical, Dental, Vision, Life Insurance, 401K with a 6% match and an annual bonus; and accrued PTO, wellness days and floating holidays.
Responsibilities:
- Design and execute molecular cloning strategies to produce new plasmid sequences for sa-mRNA production, troubleshoot the cloning process as needed.
- Amplify plasmid in E. Coli cells and perform purification using state-of-the-art automation system.
- Conduct plate-based enzymatic digestion and in-vitro transcription reactions.
- High throughput purification of linear DNA and sa-mRNA.
- Run capillary electrophoresis to analyze the quality of RNA/DNA samples.
- Participate in the development, validation and scaling up of novel assays.
- Work with automated liquid handlers to enable high throughput sa-mRNA production.
- Maintain a comprehensive Electronic Lab Notebook (ELN) and ensure all experimental procedures and documentation are compliant with highest quality and regulatory standards.
- Use GraphPad Prism and other software for data analysis and figure generation.
Qualifications:
- BS or MS in Biological Sciences or a related field
- 4+ years of experience in pharmaceutical/biotechnology labs.
- Demonstrated proficiency in molecular biology techniques including but not limited to: RNA/DNA isolations such as miniprep/maxiprep, molecular cloning (Gibson assembly/Golden Gate), agarose gel electrophoresis, PCR, etc. is required.
- Experience with Geneious, Benchling, or SnapGene.
- Bacterial transformation, plating, culturing, DNA prep, and colony screening.
- Experience in nucleic acids handling, quantitation, and analysis (e.g. Nanodrop, Qubit, Fragment analyzer, RT-qPCR, ddPCR, etc.).
- Familiarity with Hamilton or Tecan.
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Our Benefits
CSL Seqirus employees that work at least 30 hours per week are eligible for benefits effective day 1. We are committed to the wellbeing of our employees and their loved ones. CSL Seqirus offers resources and benefits, from health care to financial protection, so you can focus on doing work that matters. Our benefits are designed to support the needs of our employees at every stage of their life. Whether you are considering starting a family, need help paying for emergency back up care or summer camp, looking for mental health resources, planning for your financial future, or supporting your favorite charity with a matching contribution, CSL Seqirus has many benefits to help achieve your goals.
Please take the time to review our benefits site to see what's available to you as a CSL Seqirus employee.
About CSL Seqirus
CSL Seqirus is part of CSL Limited (ASX: CSL). As one of the largest influenza vaccine providers in the world, CSL Seqirus is a major contributor to the prevention of influenza globally and a transcontinental partner in pandemic preparedness. Learn more about CSL Seqirus.
We want CSL Seqirus to reflect the world around us
As a global organization with employees in 35+ countries, CSL embraces diversity and inclusion. Learn more about Diversity & Inclusion at CSL Seqirus.
Do work that matters at CSL Seqirus!
Watch our 'On the Front Line' video to learn more about CSL Seqirus